Challenges and opportunities for circRNA identification and delivery

Release time:2025-02-16

Impact Factor:6.2

DOI number:10.1080/10409238.2023.2185764

Affiliation of Author(s):Xiangya School of Pharmaceutical Sciences in Central South University, Changsha, Hunan, China

Journal:Critical Reviews in Biochemistry and Molecular Biology

Place of Publication:美国

Key Words:Circular RNA; analysis; biosensors; detection; identification.

Abstract:Circular RNAs (circRNAs) are evolutionarily conserved noncoding RNAs with tissue-specific expression patterns, and exert unique cellular functions that have the potential to become biomarkers in therapeutic applications. Therefore, accurate and sensitive detection of circRNA with facile platforms is essential for better understanding of circRNA biological processes and circRNA-related disease diagnosis and prognosis; and precise regulation of circRNA through efficient delivery of circRNA or siRNA is critical for therapeutic purposes. Here, we reviewed the current development of circRNA identification methodologies, including overviewing the purification steps, summarizing the sequencing methods of circRNA, as well as comparing the advantages and disadvantages of traditional and new detection methods. Then, we discussed the delivery and manipulation strategies for circRNAs in both research and clinic treatment. Finally, the challenges and opportunities of analyzing circRNAs were addressed.

Co-author:Z. Zeng, Y. Huang, C. Chen, Z. Cheng

First Author:J. Dong

Indexed by:综述论文

Correspondence Author:Q. Zhu

Discipline:Medicine

First-Level Discipline:Pharmaceutical Science

Document Type:电子版

Volume:58

Issue:1

Page Number:19-35

ISSN No.:1549-7798

Translation or Not:no

Date of Publication:2023-03-14