A DNAzyme-enhanced nonlinear hybridization chain reaction for sensitive detection of microRNA

Release time:2025-02-16

Impact Factor:4.8

DOI number:10.1016/j.jbc.2023.104751

Affiliation of Author(s):Xiangya School of Pharmaceutical Sciences in Central South University, Changsha, Hunan, China

Journal:？Journal of Biological Chemistry

Place of Publication:美国

Key Words:microRNAbranched hybridization chain reactionDNAzymeisothermal amplification techniquebioanalysis

Abstract:As a typical biomarker, the expression of microRNA is closely related to the occurrence of cancer. However, in recent years, the detection methods have had some limitations in the research and application of microRNAs. In this paper, an autocatalytic platform was constructed through the combination of a nonlinear hybridization chain reaction and DNAzyme to achieve efficient detection of microRNA-21. Fluorescently labeled fuel probes can form branched nanostructures and new DNAzyme under the action of the target, and the newly formed DNAzyme can trigger a new round of reactions, resulting in enhanced fluorescence signals. This platform is a simple, efficient, fast, low-cost, and selective method for the detection of microRNA-21, which can detect microRNA-21 at concentrations as low as 0.004 nM and can distinguish sequence differences by single-base differences. In tissue samples from patients with liver cancer, the platform shows the same detection accuracy as real-time PCR but with better reproducibility. In addition, through the flexible design of the trigger chain, our method could be adapted to detect other nucleic acid biomarkers.

Co-author:J. Dong, R. Sun, X. Zhang

First Author:X. Cao

Indexed by:Journal paper

Correspondence Author:C. Chen, Q. Zhu

Discipline:Medicine

First-Level Discipline:Pharmaceutical Science

Document Type:电子版

Volume:299

Issue:6

Page Number:104751

ISSN No.:1083-351X

Translation or Not:no

Date of Publication:2023-04-24