学位:博士学位
学历:研究生(博士)毕业
职称:教授
所在单位:湘雅基础医学院
学术荣誉: 曾获荣誉:
中南大学教授,博士生/博士后导师,现任中南大学基础医学院病理学系/湘雅医院病理科副主任、分子病理诊断中心主任。病理学国家级精品课程主讲教师、病理学国家级资源共享课程主讲教师及基础医学形态学国家级教学团队、卫计委临床重点专科骨干成员,副主编及参编《病理学》全国高等学校医学规划教材及配套教材15本,人民卫生出版社国家医学教育题库病理学学科副主编,获校级教学成果奖8项;参编专家共识2项。主持国家自然科学基金及省厅级项目10余项,作为子课题负责人参与湖南省重点研发计划项目一项,获湖南省医学学科C类项目一项(200万元)。近5年以通讯作者在PNAS、Cancer Research、Cell Discovery、Nucleic Acids Res、Signal Transduct Target Ther 、Molecular Cancer 、Advanced Science 等国际知名期刊发表论文61篇。获湖南医学科技奖一等奖一项、中华医学科技奖二等奖一项、中国抗癌协会科技奖二等奖一项。获湘雅医院医疗技术成果奖三等奖一项。湘雅医院十佳医师。
Desheng Xiao, Ph.D., professor, PhD candidate/post-doctoral supervisor, Deputy Director of Department of Pathology, Basic Medical School/Xiangya Hospital, Central South University, Director of Molecular Pathology Diagnosis Center. Main lecturer of the National Excellent Course of Pathology and the National Resource Sharing Course of Pathology, and a key member of the National Teaching Team of Basic Medical Morphology and the Key Clinical Specialty of the National Health and Family Planning Commission. Co-editor and contributor to 15 national medical planning textbooks and supplementary materials of "Pathology" published by People's Medical Publishing House, and deputy editor of the National Medical Education Question Bank of Pathology. Won 8 teaching achievement awards at the university level. Participated in the compilation of 2 expert consensus. Has presided over more than 10 projects funded by the National Natural Science Foundation and provincial and ministerial-level departments, and served as the sub-project leader of one key research and development project of Hunan Province, and obtained one C-class project of Hunan Medical Science and Technology (2 million yuan). In the past five years, 61 papers have been published as the corresponding author in international renowned journals such as PNAS, Cancer Research, Cell Discovery, Nucleic Acids Res, Signal Transduct Target Ther, Molecular Cancer, and Advanced Science. Won one first prize of Hunan Medical Science and Technology Award, one second prize of Chinese Medical Science and Technology Award, and one second prize of China Anti-Cancer Association Science and Technology Award. Won one third prize of Xiangya Hospital Medical Technology Achievement Award. Xiangya Hospital's top ten physicians.
LSH interacts with and stabilizes GINS4 transcript that promotes tumourigenesis in non-small cell lung cancer
点击次数:
影响因子:5.189
所属单位:中南大学
教研室:病理学系
发表刊物:J Exp Clin Cancer Res
关键字:GINS4; LSH; Lung cancer; mRNA stability
摘要:Background: Elucidating mechanisms in oncogenes and epigenetic modifiers are needed to gain insights into the etiology and treatment of cancer, regulation of oncogene by chromatin modifiers at post-transcriptional level is critical and remains unclear. We have investigated the role of GINS4 in NSCLC. Methods: The expression of chromatin modifier lymphoid-specific helicase (LSH) and GINS4 was assessed in tumor and normal tissue from 79 patients with NSCLC with clinical characteristics. HBE, A549, H358, and H522, PC9, 95C and 95D were cultured after overexpression or silencing of GIAT4RA. Cell proliferation assay, cell migration and invasion assays, plate colony formation assay, immunofluorescence assay, Operetta® high-content screening and analysis, Western blot analysis and Co-Immunoprecipitation (Co-IP) assay, RNA immunoprecipitation assay and tumor growth assay was used to address the potential interplay of between GINS4 and LSH, and the functional of GINS4. Results: GINS4 is highly expressed in lung cancer cells and tissues, and GINS4 expression is not association with clinical risk factors, but linked with clinical stage and lymphatic metastasis status. Higher expression of GINS4 poorly linked with overall survival in lung adenocarcinomas. Furthermore, GINS4 promoted many characteristics of tumorigenesis including cell growth, clonal formation, migration and invasion, epithelial-mesenchymal transition, tumor sphere and tumor growth in vivo. Interestingly, our results demonstrated that LSH increases GINS4 expression through binding to 3'UTR region of GINS4 and stabilizing its mRNA levels. Finally, LSH overexpression rescues GINS4 knockdown-induced features. Conclusions: GINS4 facilitates lung cancer progression by promoting key characteristics of tumor potential, and LSH epigenetically interacts with and stabilizes GINS4 transcripts.
合写作者:Na Liu, Ling Chen, Yiqun Jiang, Ying Shi, Chao Mao, Yating Liu, Min Wang, Weiwei Lai, Haosheng Tang, Menghui Gao, Desheng Xiao, Xiang Wang, Fenglei Yu, Ya Cao
第一作者:Rui Yang
论文类型:期刊论文
通讯作者:Qin Yan, Shuang Liu, Yongguang Tao
卷号:38(1)
页面范围:280
是否译文:否
发表时间:2019-01-28
收录刊物:SCI