所在实验室长期从事青光眼致病机理、视网膜缺血及再灌注损伤修复研究工作，利用急性高眼压、慢性高眼压动物模型围绕视网膜神经元，特别是视网膜节细胞的代谢变化（如细胞色素氧化酶的活性改变，琥珀酸脱氢酶的变化等）、电活动改变（如fERG、VEP）、突触可塑性及死亡方式（如凋亡、坏死、程序性坏死、焦亡）等进行了较为系统的研究，证实了自由基学说、谷氨酸兴奋性毒性损伤、神经营养因子缺乏等在上述损伤中的作用，并尝试运用神经营养因子、外泌体、干细胞移植等措施以挽救视网膜节细胞。近期研究聚焦于缺血再灌注损伤条件下视网膜神经胶质细胞在突触可塑性、神经炎症等过程中的作用及机制。上述研究依托国家自然科学基金10余项，近年发表相关研究论文情况如下：

（1）Cheng Tan, Wenjia Shi, Yun Zhang, Can Liu, Tu Hu , Dan Chen*, Jufang Huang. MiR-93-5p inhibits retinal neurons apoptosis by regulating PDCD4 in acute ocular hypertension model. Life Sci Alliance/2023 Vol. 6, No.9.

（2）Tu Hu; Shuhan Meng; Qianyue Zhang; Shuang Song; Cheng Tan; Jufang Huang; Dan Chen D ; Astrocyte derived TSP2 contributes to synaptic alteration and visual dysfunction in retinal ischemia/reperfusion injury, Cell & Bioscience, 2022, 12(1): 196-212. 

（3）Yu Zhang; Yanxia Huang; Limin Guo; Yun Zhang; Mingxuan Zhao; Fuyao Xue; Cheng Tan; Jufang Huang; Dan Chen D ; Melatonin alleviates pyroptosis of retinal neurons following acute intraocular hypertension, CNS & neurological disorders drug targets, 2020, 19(0): 1-13。

（4）Wang M, Wan H, Wang S, Liao L, Huang Y, Guo L, Liu F, Shang L, Huang J, Ji D, Xia X, Jiang B, Chen D*, Xiong K*. RSK3 mediates necroptosis by regulating phosphorylation of RIP3 in rat retinal ganglion cells. J Anat. 2020, 12. doi: 10.1111/joa.13185. [Epub ahead of print]

（5）Hu T, Wang S, Zeng L, Xiong K, Chen D*, Huang J*. Regional Expression of Act-MMP3 Contributes to the Selective Loss of Neurons in Ganglion Cell Layers following Acute Retinal ischemia/Reperfusion Injury.Curr Eye Res. 2019, 6:1-13

（6）Zuo W, Xie B, Li C, Yan Y, Zhang Y, Liu W, Huang J*, Chen D*.The Clinical Applications of Endometrial Mesenchymal Stem Cells.Biopreserv Biobank. 2018,16(2):158-164.

（7）Wang S, Hu T, Wang Z, Li N, Zhou L, Liao L, Wang M, Liao L, Wang H, Zeng L, Fan C, Zhou H, Xiong K, Huang J*, Chen D*. Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure. PLoS One. 2017, 12(9): e0185388.

（8）Zhou LH, Wang H, Luo J, Zeng LP, Xiong K, Huang JF, Chen D*. Effects of glial cells on expression of synaptophysin in rat retina with acute high intraocular pressure. Neural Regeneration Research, 2014, 9(4): 385-393

（9）Huang JF, Zhou LH, Wang H, Luo J, Zeng LP, Xiong K, Chen D*. Distribution of thrombospondins and their neuronal receptor α2δ1 in rat retina. Exp Eye Res, 2013, 111:36-49

（10）Zhou LH, Huang JF, Wang H, Luo J, Zeng LP, Xiong K, Chen D*. Expression of glutamate and GABA in synaptic plasticity of rat retina following acute high intraocular pressure. Acta Histochemia Cytochemica. 2013, 46(1):11-18

（11）Huang JF, Zhou LH, Wang H, Luo J, Xiong K, Zeng LP, Chen D*. Spatiotemporal alterations of presynaptic elements in the retina after acute high intraocular pressure, Neural Regeneration Research, 2012, 7(16): 1234-1240

（12）王慧, 罗佳, 周利红, 黄菊芳, 陈旦*. 脑源性神经营养因子对急性高眼压大鼠视网膜突触囊泡素表达的影响, 解剖学杂志,2012, 35(4): 5 473-475