Atomic Force Microscopy (AFM) as one of the cutting-edge nanotechnology has developed fast in recent years. The most recent commerial product has realized vedio-rate visualization of biomolecular processing in nanoscale. Herein, AFM is used to thread a tethered peptide chain through a subnanopore so that the translocation is systematically controlled, which can be the funfamentals in the next-generation sequencing development. In addition, capabilities of nanoindentation, resonance frequence shift weighing, conductive AFM, force spectroscopy and bio-compatible imaging, makes AFM promising in many interdisciplinary fields such as synthetic biology and virology.

Figure 1. Discriminate two H3 protein variants at single molecule level by using AFM nanomanipulation.

Z. Dong et al., ACS Nano, 2017.