On-line surface plasmon resonance-high performance liquid chromatography-tandem mass spectrometry for analysis of human serum albumin binders from Radix Astragali
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Release time:2020-08-08 Impact Factor:4.049 DOI number:10.1016/j.chroma.2013.04.015 Journal:Journal of Chromatography A Place of Publication:NETHERLANDS Key Words:SPR；HPLC–MS/MS；Human serum albumin；Radix Astragali； Isoflavonoids； Astragalosides Abstract:Development of sensitive and effective methods that meet the demand of high-throughput, high-fidelity screening of bioactive components from natural products are important to drug discovery. We describe here a novel surface plasmon resonance-high performance liquid chromatography–tandem mass spectrometry (SPR-HPLC–MS/MS) system for rapid, continuous and effective screening and identification of human serum albumin (HSA) binders from Radix Astragali. The HPLC six-port valve was used as interface through which the dissociated HSA binders from SPR was collected and injected automatically to HPLC–MS/MS for analysis. Eleven isofalvonoids and nine astragalosides have been screened and identified as the main HSA binders in ethyl acetate extract of Radix Astragali. Compared with reverse ultrafiltration assay, the developed configuration is more cost effective and reproductive because of the higher reusability and stability of immobilized HSA on SPR chip. Furthermore, the assay minimized the matrix interference in MS because of the elimination of non-binding components before HPLC separation. It is concluded that this novel technology offers new perspectives for screening and identification of active components from complex mixtures. Co-author:Junfang Guo, Qingping You First Author:[45] Yuping Zhang, Shuyun Shi*, Junfang Guo, Qingping You, Deshan Feng* Indexed by:Other Correspondence Author:Shuyun Shi, Deshan Feng Document Code:DOI：10.1016/j.chroma.2013.04.015 Discipline:地质资源与地质工程 Document Type:J Volume:1293 Page Number:92–99 ISSN No.:0021-9673 Translation or Not:no Date of Publication:2013-06-07 Included Journals:SCI -
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