Impact Factor:4.88
Journal:Ecotoxicology and environmental safety
Key Words:Biomarker, Cd-metallothionein like protein, Oxidative stress
Abstract:The biochemical responses of Bellamya aeruginosa as a dominant and widespread freshwater gastropod throughout China to waterborne cadmium (Cd) were investigated to explore the impacts of exposure concentration and duration in this potential sentinel species. After the 7 days' test of dosage-mortality relationship, gastropods were exposed for either 7 days at the LC50 (1.7mg/L), the LC10 (0.7mg/L) and 0.02mg/L Cd, or 28 days at 0.02mg/L Cd. A suite of biochemical indicators including metallothionein-like protein (MTLP), reduced glutathione (GSH), catalase (CAT), contents of tissue metal (Cd, Fe, Mn, Cu, Zn), and the compartments of these metals bound to MTLP were examined. The treatment of 0.02mg/L Cd led to the increase of Cd bound to MTLP (Cd-MTLP) levels, the decrease of GSH content, and the upregulation of CAT activity, but no induction of MTLP, indicating that the intrinsic MTLP and GSH worked together for the detoxification of Cd at the low exposure. When the exposure concentration increased, GSH was depleted severely and synthesis of MTLP was triggered, leading to a strong and significant relationship between MTLP level and Cd accumulation. At the lethal concentrations (1.7mg/L), both MTLP induction and CAT activity were inhibited while the proportion of Cd-MTLP to total Cd were increased, suggesting more intrinsic MTLP were utilized to sequester free Cd ions. Therefore, the content of Cd-MTLP in digestive glands of B. aeruginosa was recommended as a reliable biomarker for Cd contamination. Copyright © 2020 Elsevier Inc. All rights reserved.
Indexed by:Journal paper
Document Code:10.1016/j.ecoenv.2020.110365
Discipline:环境科学与工程
Document Type:J
Volume:193
Page Number:110365
ISSN No.:1090-2414
Translation or Not:no
Included Journals:SCI