Post-transcriptional regulation DPC4 gene by miR-190 in colorectal cancer cells

Release time:2020-07-12

Impact Factor:1.4

Affiliation of Author(s):中南大学

Teaching and Research Group:病理学系

Journal:J Cancer Res Ther

Key Words:Colorectal cancer, DPC4 gene, miR‑190, posttranscriptional regulation

Abstract:Objective: The objective of this study is to elucidate the regulation of the DPC4 gene by miR‑190 in colorectal cancer (CRC) cells. The present study was undertaken to determine whether the DPC4 gene is a target gene of miRNA‑190, identify target motifs and to elucidate the mechanism of regulation of DPC4 by miRNA‑190. Materials and Methods: MiR‑190 and DPC4 expression were measured in five different CRC cell lines by quantitative real‑time polymerase chain reaction (qRT‑PCR) and Western blot. The regulation of DPC4 by miR‑190 was evaluated by qRT‑PCR, Western blotting, and luciferase reporter assays in the human CRC cell line HT‑29 after treatment with miR‑190 mimics and inhibitors. Results: The DPC4 mRNA, miR‑, and DPC4 protein expression levels were highest in LS174T cells while lowest in SW480 and SW620 cells. The DPC4/miR‑190 ratio in the HT‑29 cancer cell line was the largest. MiR‑190 expression increased dramatically after treatment with miR‑190 mimics and decreased significantly after treatment with miR‑190 inhibitors. DPC4 protein expression decreased in the miR‑190 mimics transfection group when compared to the negative control (N.C.) group and increased in the miR‑190 inhibitor groups when compared to the inhibitor plus N.C. group. MiR‑190 inhibits the relative luciferase activity of psiCHECK‑2™ vector‑3’UTR compared to the N.C. group, while miR‑190 had no obvious effect on the relative luciferase activity of the psiCHECK‑2™ vector‑3’UTRmut and psiCHECK‑2™ vector transfected cells. Conclusions: The DPC4 gene might be the target gene of miR‑190, which may negatively regulate the DPC4 gene in human CRC cells by translational suppression rather than mRNA degradation.

Co-author:Zhenghao Deng, Yu Pan, Chunyan Fu, Songqing Fan, Yongguang Tao, Jianhua Zhou

First Author:Bin Xie

Indexed by:Journal paper

Correspondence Author:Desheng Xiao

Volume:14(4)

Page Number:838-843

Translation or Not:no

Date of Publication:2018-04-01

Included Journals:SCI