个人信息
教师姓名:肖德胜

学位:博士学位

学历:研究生(博士)毕业

职称:教授

所在单位:基础医学院

学术荣誉: 曾获荣誉:
个人简介

    中南大学教授,博士生/博士后导师,现任中南大学基础医学院病理学系/湘雅医院病理科副主任、分子病理诊断中心主任。病理学国家级精品课程主讲教师、病理学国家级资源共享课程主讲教师及基础医学形态学国家级教学团队、卫计委临床重点专科骨干成员,副主编及参编《病理学》全国高等学校医学规划教材及配套教材7本,人民卫生出版社国家医学教育题库病理学学科副主编,获校级教学成果奖8项;参与或主持了教育部教改项目、教育部修购专项资助项目、教育部985资助项目、国家自然科学基金及湖南省科技计划项目10项。发表本专业SCI论文90余篇。获湖南省自然科学奖三等奖一项。

Desheng Xiao, Ph.D., professor, PhD candidate/post-doctoral supervisor, Deputy Director of Department of Pathology, Basic Medical School/Xiangya Hospital, Central South University, Director of Molecular Pathology Diagnosis Center.  The main teacher of national excellent pathology course and national resource sharing course, the national teaching team of basic medical Morphology, and the main member of National Health and Family Planning Commission key clinical specialty.  Presided over or participated in 10 projects of national Natural Science Foundation of China and Natural Science Foundation of Hunan Province. Won the third prize of Natural Science award of Hunan Province (ranked the third).  Published more than 90 SCI papers in this field.  Concurrently hold the molecular pathology group member of the Chinese medical doctor association pathology branch, a member of the standing committee of the China medical equipment AI union pathology committee, the incoming chairman of hunan province medical association pathology professional committee, the chairman of hunan medical association pathology professional committee Younger Committee, the vice president of Hunan Medical Doctor Association Pathologist Branch, the vice director of hunan Clinicopathological Quality Control Center, a member of Hunan Anti-cancer Association Lung Cancer Professional Committee. and a review expert of the National Natural Science Foundation of China.



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Post-transcriptional regulation DPC4 gene by miR-190 in colorectal cancer cells


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影响因子:1.4

所属单位:中南大学

教研室:病理学系

发表刊物:J Cancer Res Ther

关键字:Colorectal cancer, DPC4 gene, miR‑190, posttranscriptional regulation

摘要:Objective: The objective of this study is to elucidate the regulation of the DPC4 gene by miR‑190 in colorectal cancer (CRC) cells. The present study was undertaken to determine whether the DPC4 gene is a target gene of miRNA‑190, identify target motifs and to elucidate the mechanism of regulation of DPC4 by miRNA‑190. Materials and Methods: MiR‑190 and DPC4 expression were measured in five different CRC cell lines by quantitative real‑time polymerase chain reaction (qRT‑PCR) and Western blot. The regulation of DPC4 by miR‑190 was evaluated by qRT‑PCR, Western blotting, and luciferase reporter assays in the human CRC cell line HT‑29 after treatment with miR‑190 mimics and inhibitors. Results: The DPC4 mRNA, miR‑, and DPC4 protein expression levels were highest in LS174T cells while lowest in SW480 and SW620 cells. The DPC4/miR‑190 ratio in the HT‑29 cancer cell line was the largest. MiR‑190 expression increased dramatically after treatment with miR‑190 mimics and decreased significantly after treatment with miR‑190 inhibitors. DPC4 protein expression decreased in the miR‑190 mimics transfection group when compared to the negative control (N.C.) group and increased in the miR‑190 inhibitor groups when compared to the inhibitor plus N.C. group. MiR‑190 inhibits the relative luciferase activity of psiCHECK‑2™ vector‑3’UTR compared to the N.C. group, while miR‑190 had no obvious effect on the relative luciferase activity of the psiCHECK‑2™ vector‑3’UTRmut and psiCHECK‑2™ vector transfected cells. Conclusions: The DPC4 gene might be the target gene of miR‑190, which may negatively regulate the DPC4 gene in human CRC cells by translational suppression rather than mRNA degradation.

合写作者:Zhenghao Deng, Yu Pan, Chunyan Fu, Songqing Fan, Yongguang Tao, Jianhua Zhou

第一作者:Bin Xie

论文类型:期刊论文

通讯作者:Desheng Xiao

卷号:14(4)

页面范围:838-843

是否译文:

发表时间:2018-04-01

收录刊物:SCI


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