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School/Department:School of Energy Science and Engineering

Education Level:With Certificate of Graduation for Doctorate Study

Business Address:中南大学 能源科学与工程学院 113

Contact Information:xuxiang@csu.edu.cn

Degree:Doctoral Degree in Engineering

Status:Employed

Alma Mater:中南大学

Xiang Xu

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Education Level:With Certificate of Graduation for Doctorate Study

Alma Mater:中南大学

Journal Publications

Current position: Home / Journal Publications
Three-Dimensional Bioprinted Scaffolds Loaded with Multifunctional Magnesium-Based Metal–Organic Frameworks Improve the Senescence Microenvironment Prompting Aged Bone Defect Repair

DOI number:10.1021/acsnano.5c03023
Journal:ACS Nano
Key Words:metal−organic framework; bone marrow mesenchymal stem cell senescence; reactive oxygen species; 3D-bioprinting; age-related bone defect repair
Abstract:Age-related bone defects cause disability and mortality in older individuals. During bone repair in older individuals, high oxidative stress and excessive inflammation in the senescent microenvironment (SME) lead to bone marrow mesenchymal stem cell (BMSC) senescence, thereby affecting bone regeneration. In this study, we prepared multifunctional magnesium (Mg) and cerium (Ce) ion-based metal–organic frameworks (MOFs) using a hydrothermal method and constructed a three-dimensional (3D) bioprinted scaffold to effectively scavenge reactive oxygen species (ROS) and sustainably release Mg2+ to improve the SME and age-related bone defect repair. Under oxidative stress, the scaffolds delayed the senescence of loaded BMSCs and promoted M2 macrophage polarization of RAW264.7 cells, further improving BMSC osteogenic differentiation. In addition, Mg2+ release promoted aldehyde dehydrogenase 3A1 expression through the activation of the nuclear factor E2-related factor 2 (Nrf2) signaling pathway, thereby delaying BMSC senescence. Adding the Wnt/β-catenin agonist SKL2001 to the scaffolds further enhanced these effects. Finally, the composite scaffolds accelerated the repair of critical-sized calvarial defects in an aged rat model. In summary, these results demonstrated the value of improving the SME for delaying BMSC senescence using multifunctional Mg-Ce-MOF and SKL2001-based 3D-bioprinting scaffolds, thereby providing an effective strategy for promoting age-related bone defect repair.
Indexed by:Journal paper
Translation or Not:no
Date of Publication:2025-06-12
Included Journals:SCI
Links to published journals:https://pubs.acs.org/doi/10.1021/acsnano.5c03023