Recent advances and prospects in the isolation by size of epithelial tumor cells (ISET) methodology

Release time:2016-04-25

Journal:Technology in cancer research & treatment

Abstract:Current technologies to identify and characterize circulating tumor cells (CTCs) and _ circulating tumor microemboli (CTMs) among hundreds of millions of leukocytes in the bloodstream can be classified into tumor-marker-dependent and -independent technology. Isolation by size of epithelial tumor cells (ISET) is a tumor-marker-independent technology, in which CTCs are isolated by filtration without use of tumor-associated markers, as a result of their large size relative to circulating blood leukocytes. ISET allows cytomorphological, immunocytological, and genetic characterization of CTCs and

Note:CTMs. It offers a number of advantages, including retention of cell morphology; non-antigen dependence; amenability of cells to further interrogation by immunolabeling, fluorescence in situ hybridization, and RNA/DNA analysis; ability to isolate CTMs; reliability. Therefore, morphological-analysis-based and antigen-independent ISET methodology can yield more accurate and objective characterization of epithelial-mesenchymal transition.

Co-author:F. L., L.; Yu, Y. C.; Wang, Ma

Indexed by:Applied Research

Discipline:临床医学

Document Type:J

Issue:2013, 12 (4),

Page Number:295-309

Translation or Not:no