HSF1 Alleviates Microthrombosis and Multiple Organ Dysfunction in Mice with Sepsis by Upregulating the Transcription of Tissue-Type Plasminogen Activator.
发布时间：2021-06-02
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影响因子：4.379
发表刊物：Thromb Haemost
关键字：heat shock factor 1 - sepsis - tissue-type plasminogen activator - coagulation - microthrombosis
摘要：Sepsis is a life-threatening complication of infection closely associated with coagulation abnormalities. Heat shock factor 1 (HSF1) is an important transcription factor involved in many biological processes, but its regulatory role in blood coagulation remained unclear. We generated a sepsis model in HSF1-knockout mice to evaluate the role of HSF1 in microthrombosis and multiple organ dysfunction. Compared with septic wild-type mice, septic HSF1-knockout mice exhibited a greater degree of lung, liver, and kidney tissue damage, increased fibrin/fibrinogen deposition in the lungs and kidneys, and increased coagulation activity. RNA-seq analysis revealed that tissue-type plasminogen activator (t-PA) was upregulated in the lung tissues of septic mice, and the level of t-PA was significantly lower in HSF1-knockout mice than in wild-type mice in sepsis. The effects of HSF1 on t-PA expression were further validated in HSF1-knockout mice with sepsis and in vitro in mouse brain microvascular endothelial cells using HSF1 RNA interference or overexpression under lipopolysaccharide stimulation. Bioinformatics analysis, combined with electromobility shift and luciferase reporter assays, indicated that HSF1 directly upregulated t-PA at the transcriptional level. Our results reveal, for the first time, that HSF1 suppresses coagulation activity and microthrombosis by directly upregulating t-PA, thereby exerting protective effects against multiple organ dysfunction in sepsis.
合写作者：Xueyan Shi,Leijing Yin,Chuyi Tan,Jia Gu,Yanjuan Liu,Caiyan Li,Gui Xiao,Ke Liu,Meidong Liu,Sipin Tan,Zihui Xiao
第一作者：Tao Li,Huan Chen
论文类型：基础研究
通讯作者：Huali Zhang(通讯作者),Xianzhong Xiao.
学科门类：医学
一级学科：基础医学
期号：2021 Jan 27.？doi: 10.1055/s-0040-1722627.
是否译文：否
发表时间：2021-01-27
收录刊物：SCI