HSF1 Is a Transcriptional Activator of IL-10 Gene Expression in RAW264.7 Macrophages.
发布时间：2021-06-02
点击次数：
发表刊物：Inflammation .
摘要：The heat shock transcription factor (HSF) is an important transactivator of the heat shock genes. Recent studies have shown that HSF1 acts as a repressor of non-heat shock genes to protect against endotoxemia. In this study, we found that heat shock treatment and HSF1 over-expression augmented the induction of interleukin (IL)-10 mRNA. Computational analysis of the mouse IL-10 promoter region showed that three potential heat shock elements (HSEs) were located at mouse IL-10 gene promoter, among which only the −387/−360 probe formed a complex with HSF1. The lack of binding of the other two HSEs to HSF1 suggested the critical role of the flanking sequences in the binding specificity of HSE to HSF1. Moreover, we showed that HSF1 overexpression transactivated mouse IL-10 gene promoter and this transcriptional activation was inhibited by the mutation of HSE in the −387/−360 region of IL-10 gene promoter using luciferase reporter assay. These findings indicate that HSF1 is a transcriptional activator of anti-inflammatory mediator IL-10 gene in RAW264.7 macrophages.
合写作者：Lingli Zhang,Fengxiu Yu,Ying Liu,Qiujuan Liang,Gonghua Deng,Guangwen Chen,Meidong Liu
第一作者：Huali Zhang（第一作者）
论文类型：基础研究
通讯作者：Xianzhong Xiao
学科门类：医学
一级学科：基础医学
期号：2012, 35(4):1558-66
是否译文：否
发表时间：2012-05-02
收录刊物：SCI