HSF1 regulates expression of G-CSF through the binding element for NF-IL6/CCAAT enhancer binding protein beta.
发布时间：2021-06-02
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发表刊物：Molecular and Cellular Biochemistry
摘要：Heat shock factor 1 (HSF1) is the major heat shock transcription factor and plays an essential role in mediating the cellular response to physiological and environmental stress. We found that LPS-induced expression of the granulocyte-colony stimulating factor (G-CSF) gene was upregulated in HSF1 knock-out (HSF1−/−) mice using a gene array. In order to determine whether and how HSF1 regulates the induced expression of G-CSF, mRNA, and protein levels of G-CSF were detected by Northern blotting and ELISA, the promoter of G-CSF was analyzed with an online transcription element search system and the transcriptional activity of the G-CSF promoter was analyzed by EMSA and a reporter gene assay. The results showed that transcription and protein secretion of G-CSF induced by LPS are both inhibited by HSF1. Three high affinity binding sites for NF-IL6/CCAAT enhancer binding protein beta, but no heat shock element, were identified in the core promoter of G-CSF. The DNA-binding capability of NF-IL6 to the G-CSF promoter was reinforced by LPS but not influenced by heat shock or HSF1. However, HSF1 was observed to bind to the binding sites of NF-IL6 in the G-CSF promoter. The transcriptional activity of the G-CSF promoter was enhanced by LPS or NF-IL6 and inhibited by HSF1 in a dose dependent manner. We conclude that HSF1 regulates expression of G-CSF through binding to the NF-IL6-binding element.
合写作者：Mingshi Yang,Qiupeng Wang,Meidong Liu,Qiujuan Liang
第一作者：Lingli Zhang
论文类型：基础研究
通讯作者：Huali Zhang（通讯作者）,Xianzhong Xiao
学科门类：医学
一级学科：基础医学
期号：2011, 352(1-2):11-7
是否译文：否
发表时间：2011-04-01
收录刊物：SCI