A vitamin-C-derived DNA modification catalysed by an algal TET homologue

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Impact Factor:48.5

DOI number:10.1038/s41586-019-1160-0

Affiliation of Author(s):中南大学生命科学学院

Teaching and Research Group:医学遗传学

Journal:Nature

Place of Publication:United Kingdom

Abstract:5-methylcytosine (5mC) is a prevalent DNA modification, and its oxidation by TET (ten-eleven translocation) dioxygenases promotes demethylation in mammals, whereas TET homologues in other eukaryotes remain poorly characterized. Here we show that CMD1 of the alga Chlamydomonas reinhardtii is a TET homologue conjugating a glyceryl moiety to the methyl group of 5mC via a carbon-carbon bond. Unlike known TET enzymes, CMD1 uses L-ascorbic acid (vitamin C) rather than 2-oxoglutarate as an essential co-substrate. The mark is reduced in CMD1 mutants with impaired high-light fitness.

Note:JCR Q1，IF 48.5

Co-author:Kaiyao Huang, Huiru Tang, Guo-Liang Xu

First Author:Jian-Huang Xue, Guodong Chen, Fuhua Hao, Hui Chen

Indexed by:Journal Article

Correspondence Author:Zhaoyuan Fang, Fang-Fang Chen, Bo Pang, Qing-Lin Yang, Xinben Wei, Qiang-Qiang Fan, Changpeng Xin, Jiaohong Zhao, Xuan Deng, Bang-An Wang, Xiao-Jie Zhang, Yueying Chu, Hui Tang, Huiyong Yin, Weimin Ma, Luonan Chen, Jianping Ding, Elmar Weinhold, Rahul M. Kohli, Wen Liu, Zheng-Jiang Zhu

Discipline:Natural Science

First-Level Discipline:Biology

Document Type:Research Article

Volume:569

Issue:7757

Page Number:581-585

ISSN No.:0028-0836

Translation or Not:no

Date of Publication:2019-05-23