中南大学
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返回首页A rapid phage-free platform for antigen-specific VNAR screening with BATCH system
发布时间：2026-05-15
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- 影响因子：
- 5.2
- DOI码：
- 10.1002/pro.70620
- 所属单位：
- 中南大学生命科学学院
- 教研室：
- 医学遗传学
- 发表刊物：
- Protein Science
- 刊物所在地：
- United States
- 关键字：
- antigen-specific single-domain antibody; bacterial two-hybrid (BATCH); Chiloscyllium plagiosum; phage-free screening; VNAR
- 摘要：
- Shark-derived variable new antigen receptors (VNARs) are promising single-domain antibodies due to their compact size, high stability and cryptic epitope access. We developed a phage-free bacterial two-hybrid (BATCH) platform for rapid antigen-specific VNAR discovery from immunized Chiloscyllium plagiosum: a Cre-immunized spleen cDNA library (~10^8) screened against pKT25c-Cre yielded two unique binders in 16 h, and AlphaFold3 modelling highlighted CDR3 dominance in the paratope. This 1- to 2-day screening phase offers a streamlined alternative for identifying stable VNARs.
- 备注：
- JCR Q1，IF 5.2
- 合写作者：
- Faxiang Li, 陈国栋
- 第一作者：
- Wenjie Tang, Jinming Hu, Yanping Zhu
- 论文类型：
- Journal Article
- 通讯作者：
- Yiqian Zeng, Jiyuan Li, Mingfeng Gu, Junjie Yang, Bo Zhong, Yike Chen, Xianxin Yan, Jianqing Chen, Xiaofeng Jiang, Zhengbing Lyu
- 学科门类：
- 理学
- 一级学科：
- 生物学
- 文献类型：
- Research Article
- 卷号：
- 35
- 期号：
- 6
- 页面范围：
- e70620
- ISSN号：
- 0961-8368
- 是否译文：
- 否
- 发表时间：
- 2026-05-15