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影响因子：

4.1

DOI码：

10.1016/j.fsi.2025.110411

所属单位：

中南大学生命科学学院

教研室：

医学遗传学

发表刊物：

Fish & Shellfish Immunology

刊物所在地：

Netherlands

关键字：

Cre; Nanobody; VNAR; Nanobody screening; White-spotted bamboo shark

摘要：

Antibodies play crucial roles in disease diagnosis and therapy. Shark-derived nanobodies (VNARs) are the smallest known antigen-binding fragments (~12 kDa), allowing efficient antigen targeting. Traditional phage display for screening shark nanobodies is limited, as not all nanobodies express well in this format. We used Cre recombinase as antigen, integrating next-generation sequencing, cell sorting, phage display and mass spectrometry; diverse strategies yielded many VNARs, and assays in mammalian cells confirmed specific binding to Cre without inhibiting its activity.

备注：

JCR Q1，IF 4.1

合写作者：

Zhengbing Lyu, 陈国栋

第一作者：

Bo Zhong, Junjie Yang, Kaixi Zheng

论文类型：

Journal Article

通讯作者：

Wenjie Tang, Lei Jia, Mingfeng Gu, Jinming Hu, Xiaofeng Jiang, Jianqing Chen

学科门类：

理学

一级学科：

生物学

文献类型：

Research Article

卷号：

163

页面范围：

110411

ISSN号：

1050-4648

是否译文：

否

发表时间：

2025-05-11