Digital droplet immunoassay based on a microfluidic chip with magnetic beads for the detection of prostate-specific antigen

Release time:2025-02-16

Impact Factor:3.1

DOI number:10.1002/jssc.202300471

Affiliation of Author(s):Xiangya School of Pharmaceutical Sciences in Central South University, Changsha, Hunan, China

Journal:Journal of Separation Science

Place of Publication:德国

Key Words:digital detection, immunoassay, magnetic beads, microfluidic chip, prostate-specific antigen

Abstract:Sensitive biomarker detection techniques are beneficial for both disease diagnosis and postoperative examinations. In this study, we report an integrated microfluidic chip designed for the immunodetection of prostate-specific antigens (PSAs). The microfluidic chip is based on the three-dimensional structure of quartz capillaries. The outlet channel extends to 1.8 cm, effectively facilitating the generation of uniform droplets ranging in size from 3 to 50 μm. Furthermore, we successfully immobilized the captured antibodies onto the surface of magnetic beads using an activator, and we constructed an immunosandwich complex by employing biotinylated antibodies. A key feature of this microfluidic chip is its integration of microfluidic droplet technology advantages, such as high-throughput parallelism, enzymatic signal amplification, and small droplet size. This integration results in an exceptionally sensitive PSA detection capability, with the detection limit reduced to 7.00 ± 0.62 pg/mL.

Co-author:J, Tian, Z. Ren, Y. Yang, Q. Gong, R. Sun, X. Zhang, W. Liu

First Author:Z. Zeng

Indexed by:Journal paper

Correspondence Author:C. Chen

Discipline:Medicine

First-Level Discipline:Pharmaceutical Science

Document Type:电子版

Page Number:1-11

ISSN No.:1615-9314

Translation or Not:no

Date of Publication:2023-10-31