DOI码：10.1093/nar/gkac368
发表刊物：Nucleic Acids Res.
摘要：The development of RNA aptamers/fluorophores system is highly desirable for understanding the dynamic molecular biology of RNAs in vivo. Peppersbased imaging systems have been reported and applied for mRNA imaging in living cells. However, the need to insert corresponding RNA aptamer sequences into target RNAs and relatively low fluorescence signal limit its application in endogenous mRNA imaging. Herein, we remolded the original Pepper aptamer and developed a tandem array of inert Pepper (iPepper) fluorescence turn-on system. iPepper allows for efficient and selective imaging of diverse endogenous mRNA species in live cells with minimal agitation of the target mRNAs. We believe iPepper would significantly expand the applications of the aptamer/fluorophore system in endogenous mRNA imaging, and it has the potential to become a powerful tool for real-time studies in living cells and biological processing.
合写作者：Jinglei Xu, Heng Tang, Shanshan Qin, Zhentian Fang, Ziang Lu, Jian Wu, Xiaocheng Weng
第一作者：Qi Wang, Feng Xiao, Haomiao Su
通讯作者：Hui Liu, Xiang Zhou
论文编号：e84
学科门类：理学
文献类型：J
卷号：50
期号：14
页面范围：e84
是否译文：否
发表时间：2022-05-17
收录刊物：SCI
发布期刊链接：https://academic.oup.com/nar/article/50/14/e84/6586873