DOI码：10.1016/j.celrep.2023.113145
发表刊物：Cell Rep.
摘要：The conserved WD40-repeat protein WDR5 interacts with multiple proteins both inside and outside the nucleus. However, it is currently unclear whether and how the distribution ofWDR5 between complexes is regulated. Here, we show that an unannotatedmicroprotein EMBOW (endogenous microprotein binder of WDR5) dually encoded in the human SCRIB gene interacts with WDR5 and regulates its binding to multiple interaction partners, including KMT2A and KIF2A. EMBOW is cell cycle regulated, with two expression maxima at late G1 phase and G2/M phase. Loss of EMBOW decreases WDR5 interaction with KIF2A, aberrantly shortens mitotic spindle length, prolongs G2/M phase, and delays cell proliferation. In contrast, loss of EMBOW increases WDR5 interaction with KMT2A, leading to WDR5 binding to off-target genes, erroneously increasing H3K4me3 levels, and activating transcription of these genes. Together, these results implicate EMBOW as a regulator of WDR5 that regulates its interactions and prevents its off-target binding in multiple contexts.
合写作者：Jianing Zhao, Zhenkun Na, Kevin Jiang, Antonella Bacchiocchi, Ken H. Loh, Ruth Halaban, Zhentian Wang
第一作者：Yanran Chen, Haomiao Su
论文类型：期刊论文
通讯作者：Xiongwen Cao, Sarah A. Slavoff
论文编号：113145
学科门类：理学
文献类型：J
卷号：42
期号：0
页面范围：113145
是否译文：否
发表时间：2023-09-26
收录刊物：SCI
发布期刊链接：https://www.sciencedirect.com/science/article/pii/S2211124723011579?via%3Dihub