TANG Li-Jun, ph.D, Professor
MAJOR: Biochemistry & Molecular Biology
MAILING ADDRESS: firstname.lastname@example.org
During Master degree train Period I was working on the mechanism of IFN-α and IFN-αcombined with GM-CSF treated CML patients. IFN-α and IFN-α plus GM-CSF can inhibit the expression of anti-apoptosis gene such as bcr-abl and bcl-2, and regulate the expression of c-myc. It is the possible mechanism that IFN-α treatment or combined treatment on CML at chronic phase can inhibit the malignant clone expansion by promoting cell death.
During Ph.D train Period I engaged in disease-related gene clone and functional analysis by EST technique and gene chips. I cloned several novel gene and carried on preliminary functional analysis of these genes which are involved in the development of human multiple myeloma.
As a post-doctor in ICGEB I engaged in protein pattern analysis of human body fluid (e.g. vaginal fluid, pleural effusion) by using 2-DE MALDI TOF/TOF MS. I was also working on the mechanism of macrophage against mycobacterium through reactive oxygen species activity, toll-like receptor activity, ER stress activity and other cellular activity.
After postdoc train I came back China and worked on macrophage’s defense mycobacterium through exosomes which was secreted from cells. Exosome is a very important carrier and has lots of function especially in charge of cells communication. The role of exosomes shed from Myco- bacterium avium sp. paratuberculosis-infected macrophages in intercellular communication processes was examined. We compared the responses of resting macrophages infected with Mycobacterium avium sp. paratuberculosis with those of resting macrophages treated with exosomes previously released from macrophages infected with Mycobacterium avium sp. paratuberculosis. Both Mycobacterium avium sp. paratuberculosis and exosomes from infected-cells enhanced the expression of CD80 and CD86 and the secretion of TNF-α and IFN-γby macrophages. Two-dimensional analysis of the proteins present in exosomes from Mycobacterium avium sp. paratuberculosis-infected macrophages compared with those from resting cells resulted in the identification by MALDI TOF/TOF mass spectrometry of the following differentially expressed proteins: two actin isoforms, guanine nucleotide-binding protein-1, cofilin-1 and peptidyl-prolyl cis-trans isomerase A.