A New Way to Discover IRESs in Pathology or Stress Conditions? Harnessing Latest High-Throughput Technologies.
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Release time:2023-02-09 Impact Factor:4.653 DOI number:10.1002/bies.201900180. Affiliation of Author(s):Xiangya Hospital, Central South University Teaching and Research Group:Department of Clinical Pharmacology Journal:Bioessays Place of Publication:United States Key Words:cancer, cap-independent translational initiation, internal ribosomal entry site, IRES trans activating factors, long-read sequencing, third-generation sequencing Abstract:The cellular internal ribosomal entry site (IRES) is one of the most important elements to mediate cap-independent translational initiation, especially under conditions of stress and pathology. However, a high-throughput method to discover IRESs in these conditions is still lacking. Here, a possible way IRES long-read sequencing based on the latest high-throughput technologies is proposed to solve this problem. Based on this design, diversity and integrity of the transcriptome from original samples can be kept. The micro-environment that stimulates or inhibits IRES activity can also be mimicked. By using long read-length sequencing technology, additional experiments that are essential for ruling out the cryptic promoters or splicing events in routine IRES identification processes can be circumvented. It is hoped that this proposed methodology may be adopted for IRES element discovery, hence uncovering the full extent of the role of IRESs in disease, development, and stress. Also see the video abstract here https://youtu.be/JuWBbMzWXS8. Co-author:Cui Jia-Jia, Guo Cheng-Xian First Author:Wang Lei-Yun Indexed by:Journal paper Correspondence Author:Yin Ji-Ye Document Code:e1900180 Discipline:Medicine First-Level Discipline:Pharmaceutical Science Document Type:J Volume:42 Issue:3 Page Number:1-10 ISSN No.:0265-9247 Translation or Not:no Date of Publication:2020-01-07 Included Journals:SCI Links to published journals:https://onlinelibrary.wiley.com/doi/full/10.1002/bies.201900180 -
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3. A New Way to Discover IRESs in Pathology or Stress Conditions Harnessing Latest High-Throughput Technologies.pdf
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