张华莉

教授 博士生导师 硕士生导师

入职时间:1997-07-09

所在单位:基础医学院

学历:博士研究生毕业

办公地点:湘雅老校区第二教学楼5楼

性别:女

联系方式:15607310187

学位:医学博士学位

在职信息:在职

主要任职:湖南省脓毒症转化医学重点实验室学术委员会副主任,湖南省病理生理学会副理事长,危重病专业委员会副主任委员,中国病理生理学会常务理事

毕业院校:中南大学

学科:基础医学

曾获荣誉:

2013-06-01  当选:  中南大学“升华学者”特聘教授

2013-06-01  当选:  湖南省自然科学杰出青年基金获得者

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KLF4 regulates the expression of interleukin-10 in RAW264.7 macrophages.

发布时间:2021-06-02

点击次数:

发表刊物:Biochem Biophys Res Commun .

关键字:Krüppel-like factor 4;Interleukin-10;Regulation;Gene expression;Inflammation

摘要:Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor. In the current study, RAW264.7 murine macrophages were treated with Escherichia coli lipopolysaccharide (LPS) to determine the expression of KLF4. A full-length cDNA or antisense oligonucleotides of KLF4 was transfected into RAW264.7 macrophages, and the expression and release of IL-10 were analyzed by RT-PCR and ELISA. The transcription and DNA binding activities of KLF4 to the IL-10 promoter were detected by the luciferase reporter and electrophoretic mobility shift assays. The results showed that treatment of RAW264.7 macrophages with LPS resulted in a dose- and time-dependent increase in KLF4 protein levels; KLF4 overexpression increased the expression of IL-10, while KLF4 inhibition decreased the expression of IL-10. The overexpression of KLF4 promoted the transcription and DNA binding activities of KLF4 to the IL-10 promoter. These results indicate that KLF4 plays an important role in regulating the expression of IL-10.

合写作者:Ying Liu,Kangkai Wang,Yansheng Feng,Meidong Liu

第一作者:Junwen Liu,Huali Zhang(第一作者)

论文类型:基础研究

通讯作者:Xianzhong Xiao

学科门类:医学

一级学科:基础医学

期号:2007, 362(3):575-81.

是否译文:

发表时间:2007-10-26

收录刊物:SCI

上一条: Kruppel样因子4对内毒素所致IL-6基因表达的调控及机制研究