Combined techniques for antigen-specific nanobody discovery in the white-spotted bamboo shark (Chiloscyllium plagiosum)

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Impact Factor:4.1

DOI number:10.1016/j.fsi.2025.110411

Affiliation of Author(s):中南大学生命科学学院

Teaching and Research Group:医学遗传学

Journal:Fish & Shellfish Immunology

Place of Publication:Netherlands

Key Words:Cre; Nanobody; VNAR; Nanobody screening; White-spotted bamboo shark

Abstract:Antibodies play crucial roles in disease diagnosis and therapy. Shark-derived nanobodies (VNARs) are the smallest known antigen-binding fragments (~12 kDa), allowing efficient antigen targeting. Traditional phage display for screening shark nanobodies is limited, as not all nanobodies express well in this format. We used Cre recombinase as antigen, integrating next-generation sequencing, cell sorting, phage display and mass spectrometry; diverse strategies yielded many VNARs, and assays in mammalian cells confirmed specific binding to Cre without inhibiting its activity.

Note:JCR Q1，IF 4.1

Co-author:Zhengbing Lyu, Guodong Chen

First Author:Bo Zhong, Junjie Yang, Kaixi Zheng

Indexed by:Journal Article

Correspondence Author:Wenjie Tang, Lei Jia, Mingfeng Gu, Jinming Hu, Xiaofeng Jiang, Jianqing Chen

Discipline:Natural Science

First-Level Discipline:Biology

Document Type:Research Article

Volume:163

Page Number:110411

ISSN No.:1050-4648

Translation or Not:no

Date of Publication:2025-05-11