Antigen specific VNAR screening in whitespotted bamboo shark (Chiloscyllium plagiosum) with next generation sequencing

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Impact Factor:4.1

DOI number:10.1016/j.fsi.2024.109661

Affiliation of Author(s):中南大学生命科学学院

Teaching and Research Group:医学遗传学

Journal:Fish & Shellfish Immunology

Place of Publication:Netherlands

Key Words:IgNAR; VNAR; Next generation sequencing; Modelling and molecular dynamics simulation; Whitespotted bamboo shark

Abstract:IgNAR shows promise in cancer and antiviral biotherapies. Its variable region (VNAR) binds antigen as strongly as IgNAR but weighs only ~12 kDa, yet antigen-specific VNAR screening remains challenging: phage display suffers from limited library coverage, low expression, unstable target protein and time-consuming procedures. Using next-generation sequencing (NGS) we identified ~3000 BAFF-specific VNARs in Chiloscyllium plagiosum vaccinated with BAFF; modelling, molecular dynamics and ELISA showed that one of the top five abundant VNARs bound BAFF more strongly than those from phage display.

Note:JCR Q1，IF 4.1

Co-author:Jianqing Chen, Guodong Chen

First Author:Bo Zhong, Shengjie Sun, Zhan Luo

Indexed by:Journal Article

Correspondence Author:Junjie Yang, Lei Jia, Kaixi Zheng, Wenjie Tang, Xiaofeng Jiang, Zhengbing Lyu

Discipline:Natural Science

First-Level Discipline:Biology

Document Type:Research Article

Volume:150

Page Number:109661

ISSN No.:1050-4648

Translation or Not:no

Date of Publication:2024-07-01